Combination of Intra-Articular and Intraosseous Injections of Platelet Rich Plasma for Severe Knee Osteoarthritis

Author: Sánchez
Year: 2016

The Regenerative Clinic's view on this research

Prof Adrian WilsonThis is a great paper from my good friend Mikel Sanchez the godfather of PRP from Victoria in northern Spain. He really has been at the heart of PRP since it’s inception 20 years ago. He shown as have many of the randomise control studies that PRP works extremely well in grade 1 and grade 2 and even moderately advanced grade 3 osteoarthritis but is not effective in grade 4. This novel treatment involves injecting the PRP into the ends of the bone using a small metal needle. The bone itself is a little bit like a Crunchy bar and is easy to inject and by injecting the bone with treating the inflammatory mediators of arthritis located within the subchondral bone or bone just beneath the surface of the joint. Using interosseous PRP he shows that grade for osteoarthritis can be treated effectively in this excellent paper”.


The aim of this study was to assess a novel approach to treating severe knee osteoarthritis by targeting synovial membrane, superficial articular cartilage, synovial fluid, and subchondral bone by combining intra-articular injections and intraosseous infiltrations of platelet rich plasma. We explored a new strategy consisting of intraosseous infiltrations of platelet rich plasma into the subchondral bone in combination with the conventional intra-articular injection in order to tackle several knee joint tissues simultaneously. We assessed the clinical outcomes through osteoarthritis outcome score (KOOS) and the inflammatory response by quantifying mesenchymal stem cells in synovial fluid. There was a significant pain reduction in the KOOS from baseline (61.55 ± 14.11) to week 24 (74.60 ± 19.19), after treatment ( = 0.008), in the secondary outcomes (symptoms, = 0.004; ADL, = 0.022; sport/rec., = 0.017; QOL, = 0.012), as well as VAS score ( < 0.001) and Lequesne Index ( = 0.008). The presence of mesenchymal stem cells in synovial fluid and colony-forming cells one week after treatment decreased substantially from 7.98 ± 8.21 MSC/L to 4.04 ± 5.36 MSC/L ( = 0.019) and from 601.75 ± 312.30 to 139.19 ± 123.61 ( = 0.012), respectively. Intra-articular injections combined with intraosseous infiltrations of platelet rich plasma reduce pain and mesenchymal stem cells in synovial fluid, besides significantly improving knee joint function in patients with severe knee osteoarthritis. This trial is registered on EudraCT with the number 2013-003982-32.

Get in touch

Find your clinic

We offer appointments nationwide and now working with partners abroad providing cutting edge Regenerative Medicine to patients in UK, Italy, Australia, Spain and Pakistan.